Abstract
DNA folding induced by polyion complexion with cationic polyelectrolytes (or catiomers) is attracting remarkable attention in the context of nucleosome formation during genome packaging and gene vector preparation. The application of block catiomers, in contrast to homocatiomers, is attractive because the spontaneously formed polyplex micellar structure can suppress the occurrence of secondary aggregation, allowing the folding of a single DNA molecule. Here, DNA is folded to form several higher-order structures, including rod-shaped, globular, and ring-shaped (toroid) structures. This review discusses the origin of this versatile higher-order structure formation by addressing the conditions and potential mechanisms underlying when and how DNA is organized into these structures upon complexation with block catiomers.
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Acknowledgments
This work was financially supported by “Precursory Research for Embryonic Science and Technology” (PRESTO) in “Molecular Technology and Creation of New Functions” from the Japan Science and Technology Corporation (JST), the Japan Society for the Promotion of Science (JSPS) through KAKENHI and Core to Core Program for A. Advanced Research Networks, and Sekisui Chemical Grant Program for Research on Manufacturing Based on Innovations Inspired by Nature.
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Osada, K. Versatile DNA folding structures organized by cationic block copolymers. Polym J 51, 381–387 (2019). https://doi.org/10.1038/s41428-018-0157-0
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DOI: https://doi.org/10.1038/s41428-018-0157-0
