Fig. 1
From: Precision synthesis of reducing-end thiol-modified cellulose enabled by enzyme selection
Phylogenetic analysis of CdPs and isolation of CsCdP. a Phylogenetic tree of selected CdPs previously characterized biochemically. Multiple-sequence alignment was performed using ClustalX (http://www.clustal.org/). CdP gene origin: Ct, C. thermocellum (also known as Ruminiclostridium thermocellum); Cc, C. cellulosi; Ta, T. africanus TCF52B; Ra, R. albus; Cs, C. stercorarium; Ce, C. cellulovorans (GenBank ID, BAV13068.1). b SDS polyacrylamide gel showing purification of CsCdP. Lane Ori shows the original enzyme stock after His-tag purification. The other lanes show the enzyme after heat treatment at the indicated temperature and time (in minutes (min))
