Fig. 3

Photographic images showing the reaction mixture of the single enzyme (CsCdP) and coupled enzyme (CbP–CdP) reactions. Synthesis reactions containing 100 mM αGlc1-P and 10 mM 1-thio-β-d-glucose in MES buffer (50 mM, pH 7.0) were carried out at 45 °C for 8 h (CsCdP reaction) and 12 h (CbP-CdP reaction). Blank reactions were performed using otherwise exactly identical conditions but lacking 1-thio-β-d-glucose