Abstract
Yeast biosurfactants have potent applications in medical, cosmeceutical, and food industries due to their specific modes of action, low toxicity, and applicability. In this study, biosurfactant-producing yeasts were screened for various industrial applications. Among them, Aureobasidium pullulans strain A11211-4-57 with potent surfactant activity from fleabane flower, Erigeron annus (L.) pers., was selected. From culture supernatant of strain A11211-4-57, five new low-surface-tension chemicals designated as pullusurfactans A–E were identified through consecutive chromatography steps, involving ODS, silica gel, Sephadex LH-20, and ODS Sep-pak cartridge columns. Based on mass and NMR measurements, structures of pullusurfactans A–E were determined as myo-inositol lipids with molecular formulae of C20H35O9, C18H32O8, C20H35O9, C24H42O9, and C18H32O8, respectively. These compounds exhibited potent biosurfactant activities (22.90, 22.40, 32.28, 25.28, and 22.44 mN/m, respectively). These results suggest that these novel biosurfactants have potential use as biosurfactants in industrial aspect.
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Acknowledgements
This study was supported by a research grant of Gyeongsangbuk-Do and a grant (no. NRF-2014R1A2A1A11052888) of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (MSIP), Republic of Korea. We thank Ms. Ji-Young Oh, Center for University Research Facility (CURF) at Chonbuk National University, for NMR measurement.
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Kim, JS., Lee, IK. & Yun, BS. Pullusurfactans A–E, new biosurfactants produced by Aureobasidium pullulans A11211-4-57 from a fleabane, Erigeron annus (L.) pers.. J Antibiot 71, 920–926 (2018). https://doi.org/10.1038/s41429-018-0089-0
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DOI: https://doi.org/10.1038/s41429-018-0089-0
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