Table 2 Antifungal, antimicrobial, and nematicidal activity activity of 1 and its analogs

Test organism	DSM	1	57	44	61	59	58	60	Reference (µg/ml)
Fungi
Alternaria solani	2947	/	/	/	/	/	/	/	16.6^c
Aspergillus fumigatus	819	/	/	/	/	/	/	/	67.0-33.3^c
Botrytis cinerea	877	/	/	/	/	/	/	/	67.0^c
Candida albicans	1665	/	/	/	/	/	/	/	4.2^c
Cryptococcus neoformans	15466	/	/	/	67.0	/	/	/	8.3^c
Fusarium oxysporum	62297	/	/	/	/	/	/	/	67.0^c
Phytophthora drechsleri^f	62679								^c
Sclerotinia sclerotiorum	1946								^c
Bacteria
Staphylococcus aureus	346	8.3	33.3	/	33.3	/	/	/	0.21-0.1^a
Staphylococcus aureus MRSA	11822	33.3	/	/	/	/	/	/	0.83^b
Bacillus subtilis	10	8.3	33.3	8.3	/	/	/	/	4.2^a
Escherichia coli	1116	/	/	/	/	/	/	/	3.3-0.83^a
Nematode
Caenorhabditis elegans	–	12.5	10	10	50	10	10	25	1.0^e

In vitro antibacterial, anti-oomycete nematicidal activity of substances 1, 57, 44, 61, 59, 58, 60 and our control drugs. For determination of antibacterial and antifungal activity, substances were dissolved in MeOH (10 mg/ml) and then further diluted to a final concentration of 1 mg/ml. 2 and 20 µl of this solution was tested against different test organisms (67.0-0.052 µg/ml final concentration). Alternatively, for determination of nematicidal activity, substances were dissolved in MeOH (1.5 mg/mL) and an aliquot thereof transferred to 24 well plate, where each well contained 1 mL of M9 buffer, to reach final concentrations in a range from 100–1 µg/mL [61]. For all assays MeOH was used as negative control and showed no activity against the selected test strains. Results of antibacterial and antifungal assays were expressed as MIC: Minimum inhibitory concentration µg/ml, while nematicidal activity was assessed as LD₅₀: lethal dose (concentration) causing over 50 % immobility of nematodes. The cell density was adjusted to 8 × 10⁶ cells/ml
N.I. no inhibition, DSMZ German collection of microorganisms and cell cultures, Braunschweig
^aOxytetracyclin hydrochloride
^bVancomycin
^cNystatin
^dKanamycin
^eIvermecitin
^fspores from agar plates were applied without justification

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