Abstract
A new antibacterial peptide named pentaminomycin C was isolated from an extract of Streptomyces cacaoi subsp. cacaoi NBRC 12748T, along with a known peptide BE-18257A. Pentaminomycin C was determined to be a cyclic pentapeptide containing an unusual amino acid, Nδ-hydroxyarginine (5-OHArg), by a combination of ESI-MS and NMR analyses. The structure of pentaminomycin C was determined to be cyclo(–l-Leu–d-Val–l-Trp–l-5–OHArg–d-Phe–). Pentaminomycin C exhibited antibacterial activities against Gram-positive bacteria including Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus. The biosynthetic gene cluster for pentaminomycin C and BE-18257A was identified from the genome sequence data of S. cacaoi subsp. cacaoi.
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Acknowledgements
This study was supported by the Japan Society for the Promotion of Science by Grants-in-aids (grant number 16K01913). The NMR spectra were recorded on Bruker Avance 600 and Avance III HD 800 spectrometers at Advanced Analysis Center, NARO.
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Kaweewan, I., Hemmi, H., Komaki, H. et al. Isolation and structure determination of a new antibacterial peptide pentaminomycin C from Streptomyces cacaoi subsp. cacaoi. J Antibiot 73, 224–229 (2020). https://doi.org/10.1038/s41429-019-0272-y
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DOI: https://doi.org/10.1038/s41429-019-0272-y
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