Abstract
A Nonomuraea sp. strain MM565M-173N2 was isolated from deep-sea sediment off the Sanriku coast, and new antibiotics were evaluated against carbapenem-resistant Enterobacteriaceae (CRE), which is a problematic group of bacteria because of their antimicrobial resistance. From 220 l of fermented broth from strain MM565M-173N2, we isolated four new antibiotics by gel filtration and HPLC, designated as sealutomicins A (1.8 mg), B (1.5 mg), C (0.8 mg), and D (0.8 mg). Their structures were determined from MS, NMR, and CD spectra. Sealutomicin A was found to be a new enediyne antibiotic, while sealutomicins B–D were aromatized products from sealutomicin A. Sealutomicin A showed strong antibacterial activity (MIC 0.05–0.2 μg ml−1) against CRE.
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Acknowledgements
This study was supported in part by grants from the Japan Society for the Promotion of Science, 26450107 to MI, and the Japan Agency for Medical Research and Development (AMED), JP18fk0108020 and JP19fk0108093 to MI and MS. The authors would like to thank Dr. N. Kumagai for optimizing the structure based on the Gaussian 16 program, and Y. Kubota and Y. Takahashi for providing technical assistance. We thank Renee Mosi, Ph.D., from Edanz Group (www.edanzediting.com/ac) for editing a draft of this paper.
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Igarashi, M., Sawa, R., Umekita, M. et al. Sealutomicins, new enediyne antibiotics from the deep-sea actinomycete Nonomuraea sp. MM565M-173N2. J Antibiot 74, 291–299 (2021). https://doi.org/10.1038/s41429-020-00402-1
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DOI: https://doi.org/10.1038/s41429-020-00402-1
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