Abstract
Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.
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Acknowledgements
This work was supported by Japan Agency for Medical Research and Development (AMED) under Grant Number JP17ae0101002 for KS and Grant-in-Aid for Young Scientists (20K15410) for TH.
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Hashimoto, T., Hashimoto, J., Kagaya, N. et al. A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster. J Antibiot 74, 354–358 (2021). https://doi.org/10.1038/s41429-021-00410-9
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DOI: https://doi.org/10.1038/s41429-021-00410-9
