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Actinoplanes kirromycinicus sp. nov., isolated from soil

The Journal of Antibiotics volume 77, pages 657–664 (2024)Cite this article

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Abstract

A novel actinomycete, designated as TPMA0078T, was isolated from a soil sample collected in Shinjuku, Tokyo, Japan. 16S rRNA gene sequence analysis indicated that strain TPMA0078T belongs to the genus Actinoplanes and is closely related to Actinoplanes regularis IFO 12514T (99.86% 16S rRNA gene sequence similarity). The spores of strain TPMA0078T were motile, and the sporangia were cylindrical. The diamino acids in the cell wall peptidoglycan of strain TPMA0078T were meso-diaminopimelic acid and 3OH-meso-diaminopimelic acid. Whole-cell sugars were glucose and mannose, with galactose as a minor component. The major cellular fatty acids identified were iso-C15:0, iso-C16:0, and anteiso-C17:0. The predominant menaquinone was MK-9(H4), and the principal polar lipid was phosphatidylethanolamine. These chemotaxonomic properties of strain TPMA0078T were consistent with those of Actinoplanes. Meanwhile, digital DNA–DNA hybridization and average nucleotide identity values showed low relatedness between strain TPMA0078T and A. regularis NBRC 12514T. Furthermore, several phenotypic properties of strain TPMA0078T distinguished it from those of closely related species. Based on its genotypic and phenotypic characteristics, strain TPMA0078T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes kirromycinicus sp. nov. is proposed. The type strain is TPMA0078T (=NBRC 116422T = TBRC 18262T).

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Funding

This study was supported in part by Grants-in-Aid for the Toho University Grant for Research Initiative Program (TUGRIP) from Toho University (to YI and YA).

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Authors and Affiliations

  1. Faculty of Pharmaceutical Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba, 274-8510, Japan

    Jiahao Zeng, Yohei Iizaka, Aya Iwai, Riku Takeuchi, Atsushi Fukumoto & Yojiro Anzai

  2. Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan

    Moriyuki Hamada & Tomohiko Tamura

Authors
  1. Jiahao Zeng
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  2. Yohei Iizaka
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  3. Moriyuki Hamada
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Correspondence to Yohei Iizaka.

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Zeng, J., Iizaka, Y., Hamada, M. et al. Actinoplanes kirromycinicus sp. nov., isolated from soil. J Antibiot 77, 657–664 (2024). https://doi.org/10.1038/s41429-024-00756-w

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