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Streptomyces secundicynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus

The Journal of Antibiotics volume 78, pages 593–599 (2025)Cite this article

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Abstract

A novel actinobacterium strain, designated HUAS ZL42T, was isolated from the leaves of Cynara scolymus. Its taxonomic position was characterized using a polyphasic method. Strain HUAS ZL42T produced spiral spore chains consisting of rod-shaped or ovoid spores with smooth surfaces on Gause’s synthetic No.1 medium. The cell wall of strain HUAS ZL42T contained ll-diaminopimelic acid as a diagnostic amino acid. Whole-cell hydrolysates contained galactose and mannose. The predominant cellular fatty acids (>10.0%) of strain HUAS ZL42T were iso-C14:0, anteiso-C15:0 and iso-C16:0. The menaquinones were MK-9 (H6), MK-9 (H4) and MK-9 (H8). The 16S rRNA gene sequence analysis showed that strain HUAS ZL42T exhibited the highest similarities to Streptomyces cyslabdanicus K04-0144T (98.6%), Streptomyces cinnabarigriseus JS360T (98.6%), Streptomyces cyaneus CGMCC 4.1671T (98.4%) and Streptomyces corchorusii DSM 40340T (98.4%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HUAS ZL42T formed an independent subclade. Phylogenetic analysis based on five housekeeping gene sequences demonstrated that strain HUAS ZL42T was most closely related to S. cyaneus CGMCC 4.1671T. However, the ANIm and dDDH between strain HUAS ZL42T and S. cyaneus CGMCC 4.1671T were 87.17% and 28.90%, respectively, far less than 96.7% and 70% cut-off points recommended for delineating Streptomyces species, suggesting that strain HUAS ZL42T was a novel Streptomyces species. On the basis of phenotypic and genotypic differentiation from tested strains, it is proposed that strain HUAS ZL42T represents a novel species of the genus Streptomyces, named Streptomyces secundicynarae sp. nov. The type strain is HUAS ZL42T (=MCCC 1K09444T = JCM 37325T).

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Acknowledgements

All authors are thanks to Zhu Zhisheng and Liu Xiupian (MCCC, Xiamen, China) for help. The NCBI accession numbers for the 16S rRNA gene sequence and genome sequence of strain HUAS ZL42T are PQ097304 and CP160403.1, respectively.

Funding

This study was supported by the Hunan Natural Science Foundation (2024JJ7300 and 2024JJ7307), 2025 Hunan Provincial College Students’ Innovation Training Program Project, the project of Hunan Provincial Education Department (23B0659 and 24A0492), Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province, Changde Key Innovation Team for wetland biology and Environmental Ecology, the Special Fund of the Comprehensive Development Studio for College Counselors in Hunan Province, and Innovation Team of Microbial Technology in Hunan University of Arts and Science (202026).

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  1. These authors contributed equally: Yu Song, Ting Tang

Authors and Affiliations

  1. Key Laboratory of Agricultural Products Processing and Food Safety in Hunan Higher Education, Hunan Provincial Engineering Research Center for Fresh Wet Rice Noodles, Science and Technology Innovation Team for Efficient Agricultural Production and Deep Processing at General University in Hunan Province, College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde, Hunan, PR China

    Yu Song, Ting Tang, Ping Mo, Zhilong Wu & Aihua Deng

  2. College of Furong, Hunan University of Arts and Science, Changde, Hunan, PR China

    Ting Tang & Ping Mo

  3. Institute of Synthetic Biology Industry, Hunan University of Arts and Science, Changde, Hunan, PR China

    Ping Mo, Fengying Liu & Aihua Deng

Authors
  1. Yu Song
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  2. Ting Tang
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  4. Zhilong Wu
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Contributions

Conceptualization, PM, FYL, and AHD; Data curation, Formal analysis, Investigation, Project administration, Software, YS, TT, and ZLW; Funding acquisition, Supervision, YS, PM, FYL, and AHD; Writing—original draft, YS and TT; Writing—review and editing, PM, FYL, and AHD; All authors have read and agreed to the published version of the manuscript.

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Correspondence to Ping Mo or Fengying Liu.

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Song, Y., Tang, T., Mo, P. et al. Streptomyces secundicynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus. J Antibiot 78, 593–599 (2025). https://doi.org/10.1038/s41429-025-00853-4

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