Fig. 2

Chemical space detected from microbial strains in this study. a Rarefaction curve displaying the number of strains (x-axis) against the number of MS/MS spectra and molecular families (y-axis); b Zoom in of rarefaction curves by annotated molecular families, annotated MS/MS spectra, unannotated molecular families and unannotated MS/MS spectra. Color code as shown in the figures; c Number of annotated spectra using GNPS spectral libraries and classified by biosynthetic pathways (NPclassifier) [65]. Bar plots showing the variation of the annotated molecules by five of the culture media. The number of annotations as shown in Fig. 1 (main text) was normalized to 1 as maximum by biosynthetic pathway. Briefly, the number of annotated MS/MS was calculated per culture condition and per biosynthetic pathway and normalized to the maximum within each pathway. Color code as shown in the figure per culture condition