Abstract
Two new 22-membered macrolides, dactylide D (1) and dactylide E (2), featuring substituted amino acid or a contiguous dipropionate unit, along with the known dactylosporolide B (3), were purified from Dactylosporangium aurantiacum ATCC 23491. Their structures were determined through comprehensive spectroscopic analysis and comparison with related congeners. Compound 1 features an N-acetylalanyl side chain, while compound 2 bears an unusual (E)-7-amino-oxohept-5-enoic acid moiety, along with C-7 oxidation and loss of the tetrahydropyran ring. NOE correlations indicated that the stereochemistry of the macrolactone cores in 1 and 2 is conserved relative to dactylide B. These findings expand the structural diversity of polyol macrolides from D. aurantiacum, underscoring the strain’s potential for natural product discovery.
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Acknowledgements
This work was supported by Scientific and Engineering Research Board (SERB), Department of Science and Technology [ECRA/2016/000788 and EEQ/2016/000268]; and Council of Scientific and Industrial Research (CSIR), New Delhi [MLP/0027]. Yedukondalu Nalli acknowledges Scientific and Engineering Research Board (SERB) for providing National Postdoctoral Fellowship (N-PDF). Sanju Singh acknowledge Council of Scientific and Industrial Research (CSIR) for JRF and SRF fellowships. Pankaj Kumar acknowledge Department of Biotechnology (DBT) for providing JRF and SRF fellowships. Central Instrumentation facility, CSIR-CSMCRI is acknowledged for acquiring MS and NMR spectral data.
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Kumar, P., Nalli, Y., Singh, S. et al. Dactylides D and E, two modified 22-membered polyol macrolides isolated from Dactylosporangium aurantiacum. J Antibiot (2026). https://doi.org/10.1038/s41429-026-00916-0
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DOI: https://doi.org/10.1038/s41429-026-00916-0
