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Kineococcus anabasis sp. nov., isolated from seeds of Anabasis brevifolia

The Journal of Antibiotics (2026) Cite this article

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Abstract

A novel actinomycete, designated as NUM-3379T, was isolated from seeds of Anabasis brevifolia collected from Urgun Soum, Dornogovi Province, Mongolia. The cells of the strain were aerobic, Gram-positive, motile, non-spore-forming, and coccus-shaped. 16S rRNA gene sequence analysis indicated that strain NUM-3379T belonged to the genus Kineococcus and showed the highest similarity to Kineococcus glutinatus DSM 26692T and Kineococcus xinjiangensis DSM 22857T, with both sharing 98.87% sequence similarity. The chemotaxonomic characteristics of the strain NUM-3379T were consistent with those of the genus Kineococcus. The diagnostic diamino acid for peptidoglycans was meso-diaminopimelic acid. The whole-cell sugars included glucose, galactose, mannose, and arabinose. The major menaquinone was MK-9(H2). The polar lipid profile was comprised of phosphatidylglycerol, diphosphatidylglycerol, and two unidentified phospholipids. The major fatty acids were anteiso-C15:0 and iso-C17:0 3-OH. In contrast, digital DNA–DNA hybridization and average nucleotide identity values revealed low relatedness between strain NUM-3379T and other type strains of the genus Kineococcus. Furthermore, strain NUM-3379T exhibited several phenotypic properties that distinguished it from other closely related species. Based on the genotypic and phenotypic data, strain NUM-3379T represents a novel species within the genus Kineococcus, for which the name Kineococcus anabasis sp. nov. is proposed. The type strain is NUM-3379T (=NBRC 117252T = TBRC 21100T).

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Acknowledgements

This research was supported in part by the Advanced Research Grant from the National University of Mongolia (grant number P2023-4571), the JICA M-JEED Project (J12A15), JST/JICA SATREPS (JPMJSA1906), and the Honda Foundation of Japan.

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  1. These authors contributed equally: Bekh-Ochir Davaapurev, Yohei Iizaka.

Authors and Affiliations

  1. School of Engineering and Technology, National University of Mongolia, Ulaanbaatar, Mongolia

    Bekh-Ochir Davaapurev, Otgonbaatar Undarmaa, Davaanyam Enkhtuul, Chimeddeleg Sod-Erdene & Javzan Batkhuu

  2. Faculty of Pharmaceutical Science, Toho University, Chiba, Japan

    Yohei Iizaka, Chiharu Najima, Jiahao Zeng & Yojiro Anzai

  3. School of Arts and Sciences, National University of Mongolia, Ulaanbaatar, Mongolia

    Enkhtaivan Bum-Erdene

  4. Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan

    Narumi Enomoto, Moriyuki Hamada & Tomohiko Tamura

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Davaapurev, BO., Iizaka, Y., Najima, C. et al. Kineococcus anabasis sp. nov., isolated from seeds of Anabasis brevifolia. J Antibiot (2026). https://doi.org/10.1038/s41429-026-00925-z

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