Fig. 2 | European Journal of Human Genetics

Fig. 2

From: Are your covariates under control? How normalization can re-introduce covariate effects

Fig. 2

The effect of applying a rank-based INT to residuals of questionnaire-type data, i.e., after regressing out covariates. All correlations referred to in this figure are Pearson (linear) correlations. a Untransformed questionnaire-type variable and its relationship with a continuous covariate. The questionnaire-type variable has a range of 5. A weak linear relationship exists between the questionnaire-type variable and covariate. b Questionnaire-type variable residuals after regressing out the relationship with the covariate. No linear relationship exists between the questionnaire-type residuals and covariate. Regressing out covariate effects has led to the separation of many tied observations, creating a covariate-based rank within the questionnaire-type variable residuals. c After the rank-based INT of questionnaire-type variable residuals, the transformed questionnaire-type variable residuals show a strong linear correlation with the covariate. This correlation is stronger and in the opposite direction to the original correlation between the untransformed questionnaire-type variable and the covariate

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