Table 1 List of Oligo used for cloning COL4A3/COL4A5 plasmids
From: New frontiers to cure Alport syndrome: COL4A3 and COL4A5 gene editing in podocyte-lineage cells
#1 | Human U6 Fw | TAAACCGCGGGAGGGCCTATTTCCCATGAT |
#2 | Human U6 Rv | TAAACTCGAGGGTACCTCTAGAGCCATTTG |
#3 | COL4A5 Cas9 Fw Primer | TAAAGGATCCCCTGGAGGGCCGAAATCAGGGGGATGG ACTATAAGGACCACGA |
#4 | COL4A5 Cas9 Rv Primer | TAAACCGCGGCCTGGAGGGCCGAAATCAGGGGGTCA GCGAGCTCTAGGAATTC |
#5 | COL4A3 Cas9 Fw Primer | TAAAGGATCCCCTGGAGAAACTGAATCACCAGGATGG ACTATAAGGACCACGA |
#6 | COL4A3 Cas9 Rv Primer | TAAACCGCGGCCTGGAGAAACTGAATCACCAGGTCAG CGAGCTCTAGGAATTC |
#7 | COL4A5 sgRNA Fw Oligo | CACCGCCTGGAGGGCCGAAATCAGG |
#8 | COL4A5 sgRNA Rv Oligo | AAACCCTGATTTCGGCCCTCCAGGC |
#9 | COL4A3 sgRNA Fw Oligo | CACCGCCTGGAGAAACTGAATCACC |
#10 | COL4A3 sgRNA Rv Oligo | AAACGGTGATTCAGTTTCTCCAGGC |
#11 | COL4A5 Donor | CCCAGGTTTACCAGGCCTCCCAGGGAATATAGGGCCTATGGGTCCCCCTGgTTTCGGCCCTCCAGGCCCAGTAGGTGAAAAAGGCATACAAGGTGTGGCAG |
#12 | COL4A3 Donor | AGACCCAGGAATTCCAGGCTTGGATAGATCAGGATTTCCTGGAGAAACTGgATCACCAGGAATTCCAGGTCATCAAGGTGAAATGGGACCACTGGGTCAA |
#13 | COL4A5 sgRNA WT Fw Oligo | gtcgCCTGGAGGGCCGAAAaCAGGGGG |
#14 | COL4A5 sgRNA WT Rv Oligo | cagaCCCCCTGgTTTCGGCCCTCCAGG |
#15 | COL4A3 sgRNA WT Fw Oligo | gtcgCCTGGAGAAACTGgATCACCAGG |
#16 | COL4A3 sgRNA WT Rv Oligo | cagaCCAGGTGATcCAGTTTCTCCAGG |
