Table 2 CasAnalyzer results on the edited bases after CRISPR/Cas9 correction
From: New frontiers to cure Alport syndrome: COL4A3 and COL4A5 gene editing in podocyte-lineage cells
Patient n (code) | Mutation | Sample | Total reads | HDR frequency | Ins/dels frequency n (%) | Wild Type reads = G (%) | Mutated reads = A (%) |
|---|---|---|---|---|---|---|---|
1 | COL4A5 c.1871G>A (p.(Gly624Asp)) | Edited cells | 110 | 58,80% | 10 (8,8%) | 95 (86%) | 14 (15%) |
2 | COL4A3 c.2567G>A (p.(Gly856Glu)) | Edited cells | 467 | 44,20% | 12 (10,4%) | 307 (66%) | 160 (34%) |
