Fig. 4: Zebrafish pgrmc1 morpholino-induced knockdown lens phenotypes.
From: A 127 kb truncating deletion of PGRMC1 is a novel cause of X-linked isolated paediatric cataract
Representative DIC lens images from pgrmc1 morpholino experiments. Wild-type Gal4s1020t/UAS:mCherry transgenic zebrafish display healthy lenses (A). Control fish injected with standard control morpholino display healthy lens (B) and minor cataract with very fine nuclear pitting (C). Fish injected with pgrmc1_MO1 (2 ng) morpholino displayed both healthy lenses (D) or cataracts of variable severity; minor nuclear central mass (E), mild nuclear central mass with fibre cell disorganisation (F) and moderate/severe nuclear density with pitting (G). Fish injected with pgrmc1_MO2 (12 ng) morpholino displayed mainly healthy lenses (H) or subtle cataracts of variable severity; minor distinct central lens density (I), minor nuclear fibre cell disorganisation (J) and moderate nuclear fibre cell disorganisation with minor pitting (K).
