Fig. 2

Genetic background, but not Pax6 genotype, influenced retinal thickness. a Confocal scans of Pax6^+/+ (Wt) and Pax6^Sey/+ (Het) retinas on three genetic backgrounds (bkgds), C57BL/6J (B6), B6129F1 (F1), and 129S1/SvImJ (129). b Quantifying retinal thickness revealed that Pax6 genotype had no influence, but bkgd significantly influenced retinal thickness. Neither Pax6 genotype nor bkgd were found to influence cell count for the (c) ganglion cell layer (GCL), (d) inner nuclear layer (INL), or (e) outer nuclear layer (ONL). Gray staining (Hoechst), inner plexiform layer (IPL), outer plexiform layer (OPL), not significant (N.S.), scale bar = 50 µm. Statistical significance was determined using ANOVA, post hoc analysis was performed using Fisher’s LSD with Bonferroni’s correction for multiple comparisons. Each dot represents an individual eye and error bars represent the mean ± the standard deviation