Fig. 2 | Gene Therapy

Fig. 2

From: Helper-dependent adenovirus-mediated gene transfer of a secreted LDL receptor/transferrin chimeric protein reduces aortic atherosclerosis in LDL receptor-deficient mice

Fig. 2The alternative text for this image may have been generated using AI.

Infection with HD-AdTTR-LDLR-TF restores low-density lipoprotein (LDL) uptake in CHOldlA7 cells. CHOldlA7 cells (a cell line deficient for the LDL receptor (LDLR)) were infected with HD-AdTTR-LDLR-TF or with a control vector and supernatants were collected; LDL uptake was determined assessing the incorporation of fluorescently labeled LDL (DiI-LDL) in CHOldlA7 cells treated with supernatant obtained from cells infected with either a control vector (a) or HD-AdTTR-LDLR-TF vector (b). DiI-LDL-positive endocytic vesicles were observed in cells treated with the LDLR-TF-containing supernatant; the incorporation of DiI-LDL was analyzed by confocal microscopy

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