Fig. 7: Demonstration of EYFP expression across different survival times after injection of anterograde AAVDJ/retrograde rAAV2retro versus anterograde AAV5/retrograde rAAV2retro. | Gene Therapy

Fig. 7: Demonstration of EYFP expression across different survival times after injection of anterograde AAVDJ/retrograde rAAV2retro versus anterograde AAV5/retrograde rAAV2retro.

From: Double viral vector technology for selective manipulation of neural pathways with higher level of efficiency and safety

Fig. 7

EYFP-native fluorescence (green color), EYFP immunofluorescence (purple color) and NeuN immunofluorescence (cyan color) in the VTA neurons are shown. Green, purple and cyan arrowheads indicate cell bodies with EYFP-native fluorescence, EYFP immunofluorescence and NeuN immunofluorescence, respectively. The intensity of native fluorescence and immunofluorescence for EYFP in the NeuN-immunopositive cell body was measured in the area enclosed with a dashed line. Scale bar = 40 µm in the merge and 12 weeks panel of AAV5/rAAV2retro (applies also to all the other panels).

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