Table 2 Validation of assay precision using UL83 and UL55 stimulation.

From: Validation of an IFN-gamma ELISpot assay to measure cellular immune responses against viral antigens in non-human primates

Monkey ID

Peptide pool

Analyst

Run

Triplicates

Intra-assay

Inter-assay

Inter-analyst

Mean SFU/well

%CV

Average triplicate %CV

Mean SFU/well

%CV

Mean SFU/well

%CV

10

UL83

A

5

80

12.9

10.9

74

9.9

78

6.3

   

6

79

11.2

     
   

10

74

14.3

     
  

B

9

60

6.1

   

71

 
   

11

75

8.2

     
   

13

78

12.9

     

16

UL55

A

5

208

7.8

5.5

212

11.3

214

1.5

   

6

197

4.7

     
   

10

238

2.2

     
  

B

9

174

4.2

   

210

 
   

11

228

7.0

     
   

13

228

6.9

     

13

UL83

A

5

389

8.3

5.9

405

10.9

433

9.6

   

6

480

2.4

     
   

10

429

5.3

     
  

B

9

372

4.1

   

378

 
   

11

358

6.0

     
   

13

404

4.1

     
  1. PBMCs from three CMV-responsive monkeys (low, medium and high responders) were stimulated with monkey CMV peptide pools UL83 or UL55 in six different runs per monkey. Data were generated by two analysts, with three runs per analyst on three different days.
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