Table 3 Validation of assay linearity.

From: Validation of an IFN-gamma ELISpot assay to measure cellular immune responses against viral antigens in non-human primates

Monkey ID

Run

Peptide pool

Coefficient of correlation (r2)

Linear range (cells/well)

16

1

UL83

0.972

25,000–200,000

16

2

UL83

0.977

8250–264,000

16

3

UL83

0.964

12,500–200,000

 

All

UL83

0.969

25,000–200,000

  1. The linear range of the assay response was determined in three different runs with varying cell densities (cells/well) under UL83 peptide stimulation.
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