Fig. 3: Efficiency of GNPs in the delivery of oligonucleotides.

a Efficiency of GNPs in the delivery of siRNA, ASO, and Ps-ASO into Hela-Luc cells for 24 h. The concentration of oligonucleotides in each well was fixed at 100 nM. The dose of EGCG in each well was 5 μg, and the weight ratio of PLL to EGCG was 1:1. GAPDH (b) and PHD2 (c) knockdown efficiency of GNPs in HeLa cells. GNPs were consisted of ASO, EGCG, and PLL. The concentration of ASO was 100 nM (1.0 μg), the weight ratio of EGCG to ASO was 10:1, and the weight ratio of PLL to EGCG was 1:1. Data are representative of three independent experiments performed with three technical duplicates in (a–c). d Cell viability of HeLa cells treated with GNPs and EGCG/PLL for 24 h. The concentrations of the materials were equal to those in (b, c). TE and LPF were tested as controls. The doses of TE and LPF in each well were both 2.0 μL. Data are representative of three independent experiments performed with five technical duplicates. **p < 0.01, ***p < 0.001 analyzed by Student’s t test.