Fig. 3: Comparison of eGFP expression driven by each of the four promoters in cultured cortical neurons transfected with AAV vector plasmids. | Gene Therapy

Fig. 3: Comparison of eGFP expression driven by each of the four promoters in cultured cortical neurons transfected with AAV vector plasmids.

From: Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters

Fig. 3

Intrinsic eGFP was measured. a Representative images of 14 DIV cortical neurons that were transfected with four different plasmids: sCAG-eGFP, hCMV-eGFP, mPGK-eGFP, hSYN-eGFP. b Quantifications of the mean eGFP intensity in neurites (df = 3(8), F = 0.23, p > 0.05, ANOVA). c Quantification of the mean eGFP intensity in the soma (df = 3(8), F = 1.39, p > 0.05, ANOVA). d Quantification of the soma area (df = 3(8), F = 0.41, p > 0.05, ANOVA). e Sholl analysis of neurite branches (df = 3(8), F = 0.52, p > 0.05, Repeated measure ANOVA, n = 3 per group). Data are shown as average ± SEM. The results are obtained from three independent experiments (n = 3) and a total of 45 transfected neurons were analysed for each condition. Images were taken with identical microscope settings between the experimental groups. Ns not statistically significant.

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