Fig. 4: Neuronal transduction efficiency and mean eGFP intensity of four promoters in the sensory-motor cortex of rats. | Gene Therapy

Fig. 4: Neuronal transduction efficiency and mean eGFP intensity of four promoters in the sensory-motor cortex of rats.

From: Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters

Fig. 4

a NeuN and eGFP staining in the cortex of AAV1-sCAG-eGFP, AAV1-hCMV-eGFP, AAV1-mPGK-eGFP and AAV1-hSYN-eGFP injected rats. b Quantification of the mean area of transduction in 40 μm thick sections (df = 3(11), F = 6.88, p < 0.01, ANOVA with Tukey’s multiple comparison test). c Quantification of the number of transduced NeuN+ cells. eGFP+ NeuN+ cells were detected using the analysis pipeline and neurons were considered as transduced when the eGFP intensity was 1.3× higher than the eGFP to background intensity ratio (df = 3(11), F = 50.4, p < 0.001, ANOVA with Tukey’s multiple comparison test). d Quantification of the mean eGFP intensity per transduced neuron (df = 3(12), F = 107, p < 0.001, ANOVA with Tukey’s multiple comparison test). The grey bars depict the averages and each dot represents the mean value of one animal. A total of four rats were analysed for the sCAG, hCMV and hSYN conditions, while three rats were analysed for the mPGK condition. Images were taken with identical microscope settings between the experimental groups. *p < 0.05; **p < 0.01; ***p < 0.001.

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