Fig. 5: Neuronal transduction efficiency and mean eGFP intensity of four promoters in the sensory-motor cortex of mice. | Gene Therapy

Fig. 5: Neuronal transduction efficiency and mean eGFP intensity of four promoters in the sensory-motor cortex of mice.

From: Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters

Fig. 5

a NeuN and eGFP staining in the cortex of AAV1-sCAG-eGFP, AAV1-hCMV-eGFP, AAV1-mPGK-eGFP and AAV1-SYN-eGFP injected mice. b Quantification of the mean area of transduction in 40 μm thick sections (df = 3(12), F = 9.29, p < 0.01, ANOVA with Tukey’s multiple comparison test). c Quantification of the number of transduced NeuN+ cells. eGFP+ NeuN+ cells were detected using the analysis pipeline and neurons were considered as transduced when the eGFP intensity was 1.3× higher than the eGFP to background intensity ratio (df = 3(12), F = 5.74, p < 0.05, ANOVA with Tukey’s multiple comparison test). d Quantification of the mean eGFP intensity per transduced neuron (df = 3(12), F = 7.58, p < 0.01, ANOVA with Tukey’s multiple comparison test). The grey bars depict the averages and each dot represents the mean value of one animal. A total of four mice were analysed for each condition. Images were taken with identical microscope settings between the experimental groups. *p < 0.05; **p < 0.01.

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