Fig. 7: Transduction efficiency of PNN-bearing interneurons depending on the promoters sCAG, hCMV, mPGK and hSYN. | Gene Therapy

Fig. 7: Transduction efficiency of PNN-bearing interneurons depending on the promoters sCAG, hCMV, mPGK and hSYN.

From: Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters

Fig. 7

a WFA (red), Aggrecan (blue) and eGFP (green) staining in the cortex of AAV1-sCAG-eGFP, AAV1-hCMV-eGFP, AAV1-mPGK-eGFP and AAV1-hSYN-eGFP injected rats and mice. b, c Quantification of the number of transduced PNN-bearing interneurons in rats (p < 0.001, Kruskal–Wallis test with Dunn’s multiple comparison test) and mice (df = 3(12), F = 10.4, p < 0.01, ANOVA with Tukey’s multiple comparison test). The grey bars depict the averages and each dot represents the mean value of one animal. Arrows highlight transduced PNN-bearing interneurons. Microscope settings for the eGFP intensity are not identical between the experimental groups in order to improve the visualisation of transduced PNN-bearing neurons; the intensity settings for WFA and aggrecan are identical throughout the representative images. *p < 0.05; **p < 0.01.

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