Fig. 4: Effect of AAV7m8 gene transfer on visual acuity and RGC in ONC. | Gene Therapy

Fig. 4: Effect of AAV7m8 gene transfer on visual acuity and RGC in ONC.

From: Rescue of retinal ganglion cells in optic nerve injury using cell-selective AAV mediated delivery of SIRT1

Fig. 4

OKR recordings demonstrate significantly decreased visual acuity in eyes of ONC mice treated with AAV2-eGFP (n = 10; experiments performed in triplicate) compared with Sham injured mice (n = 10; experiments performed in tripicate ***p = 0.002). A Treatment with AAV7m8-SIRT1 (n = 15; experiments performed in tripicate) showed a significant delay in loss of visual function in ONC (n = 10; experiments performed in tripicate) compared with Sham injured mice (n = 10; experiments performed in tripicate) (*p = 0.03) on visual acuity seen in peach area outlined in the graph. B RGC flat mount counts demonstrate significantly decreased numbers in eyes of ONC mice treated with AAV2-eGFP (n = 10; experiments performed in tripicate) compared with Sham injured mice (n = 10; experiments performed in triplicate; ***p = 0.001). Treatment with AAV7m8-SIRT1 (n = 10; experiments performed in tripicate) showed a significant increase in retinal ganglion cell counted per flat month function in ONC (n = 7) compared with control ONC mice (n = 10; experiments performed in tripicate) (*p = 0.03). C Representative RGC counts by nuclear BRN3A staining Data represented as mean ± SEM. *P < 0.05, **P < 0.01 by 1-way ANOVA with Tukey’s HSD post-test.

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