Table 1 Summary of MiniPromoters characterized in rAAV9 Ple#-intron-EmGFP-WPRE.

From: Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, corneal, endothelial, Müller glial, and PAX6 cells

Cells chosen for	Gene	Ple	MiniP size (bp)	MiniP plasmid (pEMS)	rAAV plasmid (pEMS)	Source (PMID)	Design type	Expression related to	Figure(s)
ON bipolar	PCP2	155^a	1652	1936	2115^b	KI mouse (24761428, 30765420), rAAV (26310623, 27164903)	Unaltered	Gene	1A
ON bipolar	PCP2	265^c	986	1942^b	2214^b	KI mouse (30765420)	Unaltered	Gene	1A–D and S2A
ON bipolar	PCP2	318	986	2234	2240	Ple265	Base pair modified	Negative	1A
ON bipolar	PCP2	341^c	784	2267^b	2271^b	Ple265	Cut down	Gene	1A
Cone	GNGT2	347	1197	2277	2282	Genome	New	Gene	2A–D and S2B
Cone	PDE6H	348	2025	2278	2283	Genome	New	Gene	3A
Cone	PDE6H	349^c	2005	2279^b	2284^b	Genome	New	Gene	2C, 3A–C, and S2C
Cornea	PITX3	253^c	2484	1920^b	2230^b	KI mouse (30765420), rAAV (27164903)	Unaltered	Design Source	4A–D and S2D
Endothelial BRB	CLDN5	32^c	1670	1503^b	2181^b	KI mouse (20807748, 24761428)	Unaltered	Gene	5A–E and S2E
Endothelial BRB	CLDN5	261	2963	1938^b	2218	rAAV (27164903, 31323276)	Unaltered	Gene	5A
Endothelial BRB	CLDN5	338	2567	2172	2268	Ple261	Redesigned	Gene	5A
Endothelial BRB	CLDN5	339	1973	2173	2269	Ple261	Redesigned	Gene	5A
Endothelial BRB	CLDN5	340	2700	2174	2270	Ple261	Redesigned	Gene	5A
Müller glia	NR2E1	264	3026	1941^b	2215	KI mouse (30765420), rAAV (27164903)	Unaltered	Gene	6A
Müller glia	NR2E1	315	1996	2231	2237	Ple264	Cut down	Gene	6A
Müller glia	NR2E1	316^c	1691	2232^b	2238^b	Ple264	Redesigned	Gene	6A–D and S2F
PAX6 positive	PAX6	255^a	2049	1928^b	2044^b	rAAV (27556059)	Unaltered	Gene	7A
PAX6 positive	PAX6	259^a	2087	1932^b	2048^b	rAAV (27556059)	Unaltered	Gene	7A
PAX6 positive	PAX6	328	2148	2163	2257	Ple259 with Ple255 (27556059)	Redesigned	Gene	7A
PAX6 positive	PAX6	329	2513	2164	2258	Ple259 with Ple255 (27556059)	Redesigned	Gene	7A
PAX6 positive	PAX6	330	1982	2165	2259	Genome	New	Gene	7A
PAX6 positive	PAX6	331^c	1982	2166^b	2260^b	Genome	New	Gene	7A–E and S2G

BRB blood–retina barrier, pEMS plasmid Elizabeth M. Simpson, Ple Pleiades MiniPromoter.
^aListed for comparison, but not recharacterized in this work.
^bPlasmids available at Addgene (www.addgene.org).
^cRecommended MiniPromoter.

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Table 1 Summary of MiniPromoters characterized in rAAV9 Ple#-intron-EmGFP-WPRE.

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