Fig. 1: Categorization of ChAT expressing cells in the striatum following viral targeting.
From: RNAi and chemogenetic reporter co-regulation in primate striatal interneurons
a Schematics of Lentivirus constructs. b Still image of 3D reconstruction of reporter expression using histology against the CFP or mCherry protein. Regions of at least 50% of neurons expressing the reporter were outlined on confocal images and included in the reconstruction of each injection. c Histochemistry against CFP (top) and the ChAT protein (bottom) on neighboring brain sections. Caudate and Putamen regions of the striatum were outlined to delineate regions of interest. d Visual categorization of ChAT expressing cells. Each cell positive for ChAT signal was categorized with markers (white square or triangle or circle) for either strong, medium or weak signal. e Strong, medium, and weak cells were counted (numbers on top of bars) and percentages plotted for the caudate and putamen respectively. f ChAT labeled cells in caudate and putamen (numbers on top of bars) were plotted as ratio of area in each section for either monkey. Error bars represent standard deviation in all graphs.
