Fig. 2: Morphology and function of murine AAT MΦ. | Gene Therapy

Fig. 2: Morphology and function of murine AAT MΦ.

From: Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo

Fig. 2

A Representative May–Grünwald–Giemsa staining of MΦ cytospins. Scale bar = 20 μm. B Representative flow cytometric analysis of myeloid and MΦ-specific surface marker expression by MΦ. C GM-CSF uptake from cell culture medium by MΦ. A well without cells was used as a negative control. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test. eGFP, Cbx-EFS-AAT n = 3; mock, CAG-AAT, Cbx-EF1α-AAT n = 4; no cells n = 5. D Median fluorescence intensity (MFI) fold change of pHrodo E. coli particles after phagocytosis by murine MΦ. eGFP n = 3; mock, Cbx-EFS-AAT n = 4; CAG-AAT, Cbx-EF1α-AAT n = 5. All data points and n-numbers given in Fig. 2 are derived from independent transduction experiment. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test. Bars/points represent mean ± SD. ****p ≤ 0.0001; ns not significant.

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