Fig. 4: Confirmation of viral gene delivery to liver and its effect on mouse transcript of GHR, IGF1, ALS and IGFBP3.
From: First use of gene therapy to treat growth hormone resistant dwarfism in a mouse model
a Quantification of viral transgene copies number in mouse liver. The copies numbers were normalized with mouse GAPDH housekeeping gene. Messenger RNA expression of mouse (b) GHR, (c) IGF1, (d) ALS and (e) IGFBP3 at end point in (i) male and (ii) female of uninjected GHR+/+, GHR+/−, GHR−/− and AAV-injected Laron dwarf mice with AAV8-HLP-Luc and AAV8-HLP-mGHR. The mRNA expression levels were normalized with 18S rRNA housekeeping gene. Data were presented as mean ± s.e.m. n = 3–6. Statistical significances were determined by Student’s unpaired t-test where ns indicated not significant, * indicates p < 0.05 and considered statistically significant, ** indicates p < 0.01 and considered very significant, *** indicates p < 0.001 and considered extremely significant and **** indicates p < 0.0001 and considered extremely significant.
