Fig. 2: Optimisation of DNA input and construct ratios rescues total particle titres. | Gene Therapy

Fig. 2: Optimisation of DNA input and construct ratios rescues total particle titres.

From: Enzymatically amplified linear dbDNATM as a rapid and scalable solution to industrial lentiviral vector manufacturing

Fig. 2: Optimisation of DNA input and construct ratios rescues total particle titres.The alternative text for this image may have been generated using AI.

A Mean fluorescence intensity of eGFP and B total viral particle titre (VP/mL) of LVV produced in cells transfected with decreasing total input dbDNATM and the indicated ratios of DNA:PEI. C High-throughput optimisation of construct ratios was performed using 0.5 μg/mL total input dbDNATM in 15 mL bioreactors in an automated Ambr15 cell culture system (Sartorius). Total viral particle titres from a selection of conditions are shown to demonstrate rescue of titres with reduced transfer vector. D Total viral particle titre (VP/mL) of LVV produced using plasmid at a mass construct ratio of 2:1:1:1, or dbDNATM using the molar equivalent to plasmid (Mol), or the optimised molar construct ratios of 0.5:1:3:1 and 0.5:3:3:1.

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