Fig. 3: Increasing transfer vector has little effect on low infectious and genomic titres. | Gene Therapy

Fig. 3: Increasing transfer vector has little effect on low infectious and genomic titres.

From: Enzymatically amplified linear dbDNATM as a rapid and scalable solution to industrial lentiviral vector manufacturing

Fig. 3: Increasing transfer vector has little effect on low infectious and genomic titres.The alternative text for this image may have been generated using AI.

A Infectious titres (TU/mL) of LVV generated in Fig. 2D, measured by flow cytometry of transduced VPCs 48 h post transduction. B Viral genomic RNA (vgRNA) abundance in producer cells from panel (A), measured by RT-qPCR using probes against LTR-P and eGFP. C Viral particle (VP/mL) and infectious titres (TU/mL) of LVV generated using 1 μg/mL plasmid at a mass ratio of 2:1:1:1, or 0.5 μg/mL dbDNA at the indicated molar ratios. D Genome-containing particle titre (GP/mL) of LVV from panel C measured by RT-qPCR.

Back to article page