Fig. 5: Evaluation of 3’ RS1kb in accessory constructs leads to rescue of dbDNA-derived LVV. | Gene Therapy

Fig. 5: Evaluation of 3’ RS1kb in accessory constructs leads to rescue of dbDNA-derived LVV.

From: Enzymatically amplified linear dbDNATM as a rapid and scalable solution to industrial lentiviral vector manufacturing

Fig. 5

A Infectious titre of LVV produced using 0.7 μg/mL dbDNA at a molar construct ratio of 4:1:2:1. The LV-RS1-eGFP-pA-RS1 transfer vector was used in combination with our standard accessory constructs (Std), and each accessory construct was iteratively swapped for the equivalent construct containing a 3’ RS1 element such that each construct was tested independently and in combination with all others. B Infectious titre of LVV produced using a CAR19h28z transfer vector, GagPol-RS1, Rev-RS1, and VSVg (0.7 μg/mL dbDNA at a molar ratio of 4:1:2:1). Error bars represent the standard deviation between replicates.

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