Fig. 3: High-throughput AAV tropism measurement and analysis for human ocular and cerebral organoids.
From: Multiplex viral tropism assay in complex cell populations with single-cell resolution
A AAV cell cluster tropism in transduced human ocular organoid. Data represent the cell counts that are transduced with each AAV serotype across the different cell types within the human ocular organoid. B AAV cell cluster tropism in transduced human cerebral organoid. Data represent the cell counts that are transduced with each AAV serotype across the different cell types within the human cerebral organoid. C The transduction efficiency of each AAV serotype for each cell type within the human ocular organoid is visualized as the percentage of cells transduced in a heat map. Using this method, this assay enables identification of (i) the most efficient AAV serotype for each cell cluster and (ii) the most specific AAV serotype for the target cell type of choice (i.e. lowest transduction of other non-desired cell types). D The transduction efficiency of each AAV serotype for each cell type within the human cerebral organoid is visualized as the percentage of cells transduced in a heat map plot. Using this method, this assay enables identification of (i) the most efficient AAV serotype for each cell cluster and (ii) the most specific AAV serotype for the target cell type of choice (i.e. lowest transduction of other non-desired cell types).
