Fig. 1: Generation of iCasp9-Gr- and iCasp9-Ar-expressing GMTCs and suicide gene functionality. | Gene Therapy

Fig. 1: Generation of iCasp9-Gr- and iCasp9-Ar-expressing GMTCs and suicide gene functionality.

From: RapaCaspase-9-based suicide gene applied to the safety of IL-1RAP CAR-T cells

Fig. 1

A Retroviral transduction efficiency of T cells determined by flow cytometry. T cells from HDs were genetically modified. Transduction efficiency was analyzed on day 7 after the beginning of production. Mean ± SD of three independent experiments. B Representative dot plots for AP1903-induced killing determined by flow cytometry. C Cell death percentages of cells expressing iCasp9-G measured 24 h after 10 nM AP1903 exposure. Normalized to control cells (untreated cells). Cell death was evaluated by Annexin-V/7-AAD labeling and gating on CD3+/CD19+ cells (−Gr) or CD3+/CD34+ cells (−Ar) by flow cytometry. Mean ± SD of three independent experiments. ****P < 0.0001 with unpaired Student t test.

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