Fig. 3: Double hairpins at the 5’ end interfere with cap-dependent translation of mCherry. | Gene Therapy

Fig. 3: Double hairpins at the 5’ end interfere with cap-dependent translation of mCherry.

From: A capless hairpin-protected mRNA vaccine encoding the full-length Influenza A hemagglutinin protects mice against a lethal Influenza A infection

Fig. 3

a mRNA vector schematics; © in vectors 1C and 2C indicates that the transcript has been capped using an Anti-Reverse Cap Analog (ARCA); A and B: individual hairpin sequences that comprise the double hairpin in mRNA vectors 2 and 2C. b eGFP and mCherry expression in MDCK cells 12 h after mRNA transfection with equimolar amounts of each vector (n = 4 independent experiments).

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