Fig. 1: Cultured ARPE-19 cells exhibit normal morphology and ploidy. | Gene Therapy

Fig. 1: Cultured ARPE-19 cells exhibit normal morphology and ploidy.

From: CRISPR targeting of SNPs associated with age-related macular degeneration in ARPE-19 cells: a potential model for manipulating the complement system

Fig. 1

A Phase contrast images of one month differentiated ARPE-19 cells in culture display typical retinal pigment epithelium (RPE) morphology, characterised by a uniform monolayer and distinct tight-junctions. Images taken at 10X and 20X magnifications. B comparison of freshly plated ARPE-19 cells 99-day differentiated, non-passaged cells, cells were stained for Zo-1 (green) to highlight tight-junctions, and Hoechst 3334 (blue) as a nuclear counterstain. C Multicolour fluorescence in situ hybridisation (MFISH) analysis of sub-confluent ARPE19 cells using fluorescent probes against human chromosomes. This analysis revealed a normal modal number of n = 46, with a translocation between chromosomes 15 and 19, and part of chromosome 13 translocated to chromosome 22.

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