Fig. 2: CAR-mediated phagocytosis is dependent on intra- and extracellular domains for selectivity and function.
From: Expression of anti-amyloid CARs in microglia promotes efficient and selective phagocytosis of Aβ1‒42
A schematic of CAR constructs used for testing of Aβ selectivity. B summary of flow cytometric data showing fold MFI values for the mScarlet+ (CAR high) population versus the mScarlet- (CAR low) population (n = 6 per group) relative to viral control group (mScarlet). C schematic of CAR constructs used for testing ICD dependence. D Summary of flow cytometric data showing fold MFI values for the mScarlet+ (CAR high) population versus the mScarlet- (CAR low) population (n = 4‒6 per group) relative to viral control group (mScarlet). E Kinetics of FAM-Aβ42 phagocytosis and effect of the actin polymerization inhibitor cytochalasin D (CytoD, 5 µM). *P < 0.05 **P < 0.01, ***P < 0.001. Adu; scFv derived from aducanumab, Lec; scFv derived from lecanemab, Don; scFv derived from donanemab, Pras; scFv derived from prasinezumab. ITAM/ITIM; immunoreceptor tyrosine-based activation/inhibitory motif.
