Table 1 Transduction efficiency data obtained in flow cytometry analyses of hiMG and primary mouse microglia.
From: Expression of anti-amyloid CARs in microglia promotes efficient and selective phagocytosis of Aβ1‒42
Figure 1 | Range (%) | Mean | SD | n |
|---|---|---|---|---|
Non-transduced | 0 | 0.00 | 0.00 | 4 |
mScarlet | 76,9–79,2 | 78.23 | 0.90 | 4 |
TREM2 CAR | 16–20,5 | 17.95 | 1.56 | 6 |
DAP12 CAR | 37,8–39,6 | 38.62 | 0.71 | 6 |
SIRPB1 CAR | 16,4–17,9 | 17.17 | 0.49 | 6 |
FcRγ CAR | 27,2–29,2 | 27.83 | 0.68 | 6 |
FcγRI CAR | 37,1–40,7 | 38.90 | 1.33 | 6 |
FcγRIIa CAR | 46,2–48,8 | 47.08 | 0.84 | 6 |
FcγRIIIa CAR | 45–47,4 | 46.17 | 0.99 | 6 |
Fig. 2B | Range (%) | Mean | SD | n |
|---|---|---|---|---|
Non-transduced | 0,18–0,28 | 0.22 | 0.03 | 6 |
mScarlet | 46–53 | 48.10 | 2.31 | 6 |
Adu FcRγ CAR | 15,6–16,4 | 16.12 | 0.25 | 6 |
Don FcRγ CAR | 46,3–50,1 | 48.00 | 1.22 | 6 |
Lec FcRγ CAR | 18,9–24,9 | 23.36 | 2.06 | 6 |
Pras FcRγ CAR | 45,6–48,8 | 47.82 | 1.13 | 6 |
Fig. 2D | Range (%) | Mean | SD | n |
|---|---|---|---|---|
Non-transduced | 2,19–2,64 | 2.47 | 0.17 | 4 |
mScarlet | 77,7–79,5 | 78.50 | 0.68 | 4 |
FcRγ | 10,7–13,6 | 12.52 | 0.94 | 6 |
FcγRIIb ICD | 19,6–23 | 20.80 | 1.16 | 6 |
Fig. 2E | Range (%) | Mean | SD | n |
|---|---|---|---|---|
mScarlet | 60,7–67,1 | 64.23 | 2.65 | 3 |
mScarlet CytoD | 66,4–69,3 | 68.03 | 1.21 | 3 |
FcRγ | 19,7–23,8 | 21.10 | 1.91 | 3 |
FcRγ CytoD | 18,6–20,5 | 19.70 | 0.80 | 3 |
Fig. 3 | Range (%) | Mean | SD | n |
|---|---|---|---|---|
Non-transduced | 0 | 0.00 | 0.00 | 4 |
mScarlet | 80,1–91,8 | 85.88 | 4.72 | 4 |
mFcRγ CAR | 50,3–64 | 56.87 | 4.93 | 6 |
mFcγRI CAR | 47,3–59,9 | 52.62 | 4.18 | 6 |
