Table 1 Experimental details of ultrasound-guided in utero injection procedures in piglets.

From: Transabdominal ultrasound guided AAV9-GFP delivery in fetal pigs: a translational and minimally invasive model for in utero fetal gene therapy

 

Experiment

 

SOW1

SOW2

SOW3

SOW4

GA (days)

80

108

108

108

Injected Fetuses

2

2

2

3

Dose (vg/Kg)

4.5 × 1012

5 × 1013

(1x) Saline (1x) 2.5 × 1013

(1x) Saline (2x) 1.5 × 1012

Route

(1x) u.v.

(1x) i.c.

i.c.

i.c.

(1x) u.v.

(2x) i.c.

Volume (ml)

1

2

2

1

Surgical duration (min)

30

20

20

45

Delivery day

96

115

115

115

Litters

15

16

11

16

  1. GA gestational age, vg vector genomes, i.c. Intracardiac, u.v. umbilical vein.
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