Fig. 2: Library distribution throughout the selection process.
Random AAV display peptide libraries at a dose of between 7.2 × 1011 genomic particles (gp)/ animal (=1.6 × 1012 gp/kg bodyweight) and 6 × 1012 gp/animal (=2.4 × 1013 gp/ kg bodyweight) were infused into the saphenous vein of adult male marmoset monkeys. The number of AAV library genomes recovered from different organs was quantified by qPCR 2 d after i.v. injection. Recovered AAV genomes were normalized to the number of injected particles and the animals’ body weight, indicated as the percentage of injected particles per kg bodyweight recovered from 1 µg tissue DNA. A AAV library biodistribution during the 1stscreening campaign (two-steps libraries) over the course of four consecutive selection rounds. B AAV library biodistribution during the 2ndscreening campaign (one-step libraries) over the course of two consecutive selection rounds. C Fold-change in genomic particles recovered from individual organs between consecutive selection rounds of the first screening campaign (two-steps libraries). D Fold-change in genomic particles recovered from individual organs between consecutive selection rounds of the second screening campaign (one-step libraries).
