Fig. 3: Performance gain of the CSC assay generalizes to preclinical model species.
From: Overcoming matrix effects in AAV neutralization assays with a constant serum concentration approach
Throughout the figure, Constant refers to the CSC method, where total serum remains fixed at 10%, while Variable refers to the VSC method, where total serum concentration increases with less dilution. A–F Transduction curves for sera sampled from preclinical subjects tested against three AAV serotypes (AAV1, AAV5, AAV9). (A–C) shows data from cats (n = 4), while (D–F) shows data from rhesus macaques (n = 2). Each color represents an individual subject within each species; the same color denotes the same subject across panels. Solid curves indicate results from the Constant assay, while dashed curves represent results from the Variable assay. Vertical error bars indicate standard deviations from the mean. The horizontal gray dashed line at 50% transduction efficiency indicates the neutralization threshold. For samples that cross below this threshold, ND50 dilutions are marked by colored vertical dashed arrows pointing to horizontal bars that denote 95% credible intervals for the ND50 estimates. Samples without arrows did not reach 50% neutralization within the tested dilution range. The x-axis represents 2-fold serum dilutions, while the y-axis shows transduction efficiency as a percentage of antibody-free controls.
