Fig. 2: Stability changes of AAV8 under pH stress. | Gene Therapy

Fig. 2: Stability changes of AAV8 under pH stress.

From: Comprehensive forced degradation study revealing diverse chemical and physical degradation pathways of AAV8

Fig. 2

AAV8 samples were dialyzed into a glycine-based buffer at pH 3.0 and a Bis-Tris Propane-based buffer at pH 9.0, respectively. The samples were subsequently analyzed and compared to a control sample at pH 7.3. A In vitro protein expression (n = 3). B Quantitation of Asn deamidation. C N263 (PDB:2QA0), highlighted in red, is positioned on the capsid surface and is susceptible to deamidation. D Quantitation of Asp isomerization (n = 1). E Average hydrodynamic diameter (n = 5, technical replicates). F Monomeric capsid recovery (n = 1). G Normalized vector genome titer (n = 4, technical replicates). H Normalized free DNA (n = 4, technical replicates). I VP ratio (n = 2, technical replicates).

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