Fig. 3: Stability changes of AAV8 under thermal stress at 40°C and 45°C. | Gene Therapy

Fig. 3: Stability changes of AAV8 under thermal stress at 40°C and 45°C.

From: Comprehensive forced degradation study revealing diverse chemical and physical degradation pathways of AAV8

Fig. 3

A Determination of Tonset and Tm by DSF using SYBR® gold dye. B In vitro protein expression (n = 3). Quantitation of C Asp isomerization, D Asn deamidation, and E Met oxidation (n = 1). F D220 (PDB:2QA0), highlighted in red, is oriented towards the interior of the capsid, near the five-fold axis pore. G Average hydrodynamic diameter (n = 5, technical replicates). H Monomeric capsid recovery (n = 1). I Normalized vector genome titer (n = 4, technical replicates). J Normalized free DNA (n = 4, technical replicates). K VP ratio (n = 2, technical replicates).

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