Table 1 Summary of stress conditions utilized in AAV8 forced degradation study.
Type of stress | Stress condition | Duration |
|---|---|---|
Oxidation | • 1 ppm, 1000 ppm H2O2 | Up to 1 week at 25 °C |
Temperature | • 40 °C, 45 °C | Up to 4 weeks |
pH | • pH 3.0, pH 9.0 | Up to 1 week at 25 °C |
Freeze-thaw | • Freeze at -80°C, thaw at room temperature | Up to 4 cycles |
Agitation | • Vortex at 1000 rpm | Up to 120 minutes |
