Table 2 Summary of AAV8 degradation pathways under stress conditions.
Degradation pathways and analytical panel | Oxidative stress | Low pH | High pH | Thermal stress | Agitation | Freeze-thaw | |
|---|---|---|---|---|---|---|---|
LC-MS/MS | Deamidation (Asn) |  |  | √ | √ | Not determined | Not determined |
Oxidation (Met) | √ |  |  | √ | Not determined | Not determined | |
Isomerization (Asp) |  | √ |  | √ | Not determined | Not determined | |
Microfluidic electrophoresis | Capsid fragmentation | √ | √ | √ | √ |  |  |
ddPCR & Free DNA analysis | Genome DNA release | √ | √ |  | √ | Not determined | Not determined |
DLS | Capsid aggregation |  | √ |  | √ |  |  |
