Fig. 5: Sequence and structural comparison of Mac-1 protein in SARS-CoV-2 to other CoVs and Mac-1 capacity to bind ADPr.
From: Evolutionary and structural analysis of SARS-CoV-2 specific evasion of host immunity
a Comparison of Mac-1 sequence of SARS-CoV-2 (YP_009725299.1) to that of MERS-CoV (PDB entry: 5HOL), SARS-CoV (PDB entry: 2FAV), and bat-RatG13 (QHR63299.1). Secondary structure elements are depicted in red at the top of the alignment. Alignment positions with fixed substitutions within Mac-1 that are specific to SARS-CoV-2 are highlighted in light blue columns with asterisk (*) symbol. Amino-acid residues that form a hydrogen bond with ADP-ribose (ADPr) are highlighted as yellow, pink, and green for SARS-CoV-2, SARS-CoV, and MERS-CoV, respectively. b–d Structures of the Mac-1 complex with ADPr. In each case, amino-acid residues that form a hydrogen bond with ADPr molecule are shown. e–g Comparison of interactions with ADPr in the Mac-1 adenine pocket of SARS-CoV-2, MERS-CoV, and SARS-CoV. Hydrogen bonds are shown as yellow dashed lines and bond lengths are given in Å units.
