Fig. 5: Influence of TIMP-1 on MHC-I expression levels in myeloid DCs. | Genes & Immunity

Fig. 5: Influence of TIMP-1 on MHC-I expression levels in myeloid DCs.

From: TIMP-1 is an activator of MHC-I expression in myeloid dendritic cells with implications for tumor immunogenicity

Fig. 5

A Schematic representation of the functional study to evaluate TIMP-1 impact on MHC-I levels in myeloid DCs. Flow cytometry analysis of surface MHC-I expression on primary myeloid DCs under different conditions: 1:4 EV-TCM from B16F10 cells, recombinant TIMP-1, or a combination of both. B left Hierarchical Stochastic Neighbor Embedding (HSNE) plots for unsupervised clustering of the three distinct MHC-I expression patterns: high (red), intermediate (green), and low (blue) expression levels. B right Single-cell density analysis within each MHC-I cluster for each experimental group. Normalized frequencies of live myeloid DCs across low (C, left), mid (C, middle), and high (C, right) MHC-I expression levels. Three independent experiments are shown, mean ± SEM from combined biological replicates, two-tailed unpaired t test (***p < 0.001; *p < 0.05; ns: not significant).

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